Research Interests

• The cell signaling problem
• Developing models for immunoreceptor signaling (BCR, TCR, and high-affinity receptor for IgE)
• Analysis of ligand-receptor binding
• Development of analysis tools for the BIAcore instrument
• Biosensor design

I have a strong interest in the dynamics of cell surface receptors and how they mediate cell signaling events. My research is focused on receptors that play key roles in the immune response-the cytokine receptors and the multisubunit immune recognition receptors. I am a member of the T-10/T-12 Signaling Team. At present we are building a detailed predictive model of the early cell signaling events mediated by the high affinity receptor for IgE, a major participant in allergic reactions of the immediate type. I also have an interest in biosensors and have helped develop a computer model of the flow based optical biosensor, BIAcore. We use the computer model to simulate BIAcore experiments and test methods for extracting fundamental rate constants from experiment. All my work is done in close collaboration with experimental colleagues.

Selected Publications

1. W. S. Hlavacek, J. R. Faeder, M. L. Blinov, A. S. Perelson and B. Goldstein (2003). The complexity of complexes in signal transduction. Biotech. Bioeng. 84:784-794.

2. J. R. Faeder, W. S. Hlavacek, I. Reischl, M. L. Blinov, H. Metzger, A. Redondo, C. Wofsy and B. Goldstein (2003). Investigation of early events in FcεRI-mediated signaling using a detailed mathematical model. J. Immunol. 170: 3769-3781.

3. B. Goldstein, J. R. Faeder, W. S. Hlavacek, M. L. Blinov, A. Redondo, and C. Wofsy (2002), Modeling the Early Signaling Events Mediated by FcεRI. Mol. Immunol. 38:1213-1219.

4. D. Coombs, A. M. Kalergis, S. G. Nathenson, C. Wofsy and B. Goldstein (2002). Activated TCR remain marked for internalization after dissociation from peptide-MHC. Nature Immunol. 3:926--931. [Commentary pp. 895-896]

5. C. Wofsy, D. Coombs and B. Goldstein (2001). Calculations show substantial serial engagement of T cell receptors. Biophys. J. 80:606-612.

6. W. S. Hlavacek, A. Redondo, H. Metzger, C. Wofsy and B. Goldstein (2001). Kinetic proofreading models for cell signaling predict ways to escape kinetic proofreading. Proc. Natl. Acad. Sci. USA 98:7295-7300. [Commentary pp. 6989-6990]

7. C. Wofsy, B.M. Vonakis, H. Metzger & B. Goldstein (1999) One Lyn molecule is sufficient to initiate phosphorylation of aggregated high-affinity IgE receptors. Proc. Natl. Acad. Sci. USA 96, 8615-8620. [MEDLINE abstract/Full Text]

8. D.G. Myszka, X. He, M. Dembo, T. Morton & B. Goldstein (1998). Extending the range of rate constants available from BIACORE: Interpreting mass transport influenced binding data. Biophys. J. 75, 583-594. [MEDLINE abstract/Full Text]

9. C. Torigoe, B. Goldstein, C. Wofsy & H. Metzger (1997) Shuttling of initiating kinase between discrete aggregates of the high affinity receptor for IgE regulates the cellular response. Proc. Natl. Acad. Sci. USA 94, 1372-1377. [MEDLINE abstract/Full Text]

10. C. Wofsy, C. Torigoe, U.M. Kent, H. Metzger & B. Goldstein (1997) Exploiting the difference between intrinsic and extrinsic kinases: Implications for regulation of signaling by immunoreceptors. J. Immunol 159, 5984-5992. [MEDLINE abstract]